Hi jrmoncrieff, it’s great that you’re showing an interest in my project and asking good questions!
I get my protein by expressing it from the gene. So what I have is a circular piece of DNA called a vector, and in that circle is the DNA which codes for my protein. What I do is mix many copies of this vector with E. coli, a type of bacteria. The bacteria will take up the vector and start making protein by reading from the sequence I have given them. The bacteria multiply pretty quickly so you end up with a lot of them, making a lot of copies of the protein. What you can then do is break open the bacteria and purify the protein out in a number of steps. Then you have the protein that you can use for experiments like Western blotting. You can look that up (I’m sure Wiki has a good explanation) or I can explain to you how I do it if you like.
Hi jrmoncrieff, it’s great that you’re showing an interest in my project and asking good questions!
I get my protein by expressing it from the gene. So what I have is a circular piece of DNA called a vector, and in that circle is the DNA which codes for my protein. What I do is mix many copies of this vector with E. coli, a type of bacteria. The bacteria will take up the vector and start making protein by reading from the sequence I have given them. The bacteria multiply pretty quickly so you end up with a lot of them, making a lot of copies of the protein. What you can then do is break open the bacteria and purify the protein out in a number of steps. Then you have the protein that you can use for experiments like Western blotting. You can look that up (I’m sure Wiki has a good explanation) or I can explain to you how I do it if you like.
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